The Gotham Knights were a football team from Gotham City.
In one of the Scarecrow's plans to gain money, he bet on games he rigged by striking fear into the star players. He attempted to poison the Knights' quarterback, Mitch Nole, for a game against the Colonials, but he was brought to a halt by Batman and Robin.
- The term "Gotham Knights" has also been used to refer to the Bat-family of crime fighters.
- In the spin-off comic series Batman Adventures, the Gotham Knights were also a basketball team, and in Superman Adventures, a baseball team playing against the Meteors for the World Series.